Posted on: April 3, 2003
Hair stem cells are located in the bulge area. These cells are critical for hair cloning, since they can give rise to all types of cells present in mature hair.
To be able to separate bulge stem cells for further enrichment in the cell cultures very important. CD34 is a marker for progenitor cells of the blood. It was found to be also present in on hair follicle bulge cells.
Current investigation confirmed that CD34 marker indeed is present on bulge cells in mice. Other stem cells markers, such as keratin 15 were also present in site of CD34. Authors used antibody for CD34 to segregate live bulge cells. cell cycle status analysis showed characteristics of stem cells (slowly cycling cells). It was shown, that in cell culture these CD34+ cells form larger colonies than CD34-cells, indicating a higher proliferative potential (stem cells properties).
Authors state in their article, that "...this work is the first to demonstrate that CD34 is a specific marker of bulge cell keratinocytes in the cutaneous epithelium. Furthermore, the use of this marker facilitates isolation of live epithelial cells with stem and progenitor cell characteristics, potentially providing a tool for the study of carcinogen target cells, gene therapy, and tissue engineering applications..."
Based on: Trempus CS et al., Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34. J Invest Dermatol 2003 Apr;120(4):501-11 |
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